Facility Mission
The mission of the Proteomics and Metabolomics Facility is to serve as an enabling resource for research and development programs at Colorado State University.
We strive to build instrumental capabilities that exceed the normal resources of individual research programs, and make those technologies available as a shared resource. We also aim to provide an environment rich in expertise and educational resources, and to foster collaboration across the CSU community and beyond.
Facility Publications (PMF personnel in bold)
Fujimoto S; Seebart C; Guastafierro T; Prenni J; Caiafa P; Zlatanova J “Proteome analysis of protein partners to nucleosomes containing canonical H2A or the variant histones H2A.Z or H2A.X" Biological Chemistry, 2011 In Press.
Machain-Williams C., Mammen MP, Zeidner NS, Beaty BJ, Prenni JE, Nisalak A, Blair CD “Association of Human Immune Response to Aedes aegypti Salivary Proteins with Dengue Disease Severity”,Parasite Immunol 2011 Oct 13
Heuberger AL; Lewis MR; Chen M-H; Brick MA; Leach JE; Ryan EP; “Metabolomic and Functional Genomic Analyses Reveal Varietal Differences in Bioactive Compounds of Cooked Rice.” PLoS ONE, 2010. 5(9): e12915.
Al-Mubarak R, Vander Heiden J, Broeckling CD, Balagon M, Brennan PJ, Vissa VD. "Serum metabolomics reveals higher levels of polyunsaturated Fatty acids in lepromatous leprosy: potential markers for susceptibility and pathogenesis." 2011 PLoS Negl Trop Dis. Sep;5(9):e1303.
Ryan E.P.; Heuberger A.L.; Weir T.L.; Barnett B.; Broeckling C.D.; Prenni J.E. “Rice Bran Fermented with Saccharomyces boulardii Generates Novel Metabolite Profiles with Bioactivity.” 2011. J Agric Food Chem. 59(5): 1862–1870.
Kruh, N.; Troudt, J.; Izzo, A.; Prenni, JE; Dobos, KM. "Portrait of a Pathogen: The Mycobacterium tuberculosis Proteome In Vivo" 2010, PLoS One. Nov 11;5(11): e13938.
Seebart, C.; Prenni, J.; Tomschik, M.; Zlatanova, J. “New Nuclear Partners for Nucleosome Assembly Protein 1: Unexpected Associations” 2010, Biochem. Cell Biol. 88(6): 927-936.
Goldstrohm D.A.; Broeckling C.D.; Prenni J.E.; Curthoys N.P “Importance of Manual Validation for the Identification of Phosphopeptides using a Linear Ion Trap Mass Spectrometer” 2010, J Biomol Tech., 22(1): 10–20.
Szerlong HJ; Prenni JE; Nyborg JK; Hansen JC. “Activator-dependent p300 acetylation of chromatin in vitro: enhancement of transcription by disruption of repressive nucleosome-nucleosome interations". 2010 J Biol Chem. Aug 18.
Mehaffy, C.; Hess, A.;Prenni, JE.;Mathema, B.;Kreiswirth, B.;Dobos, K.M. "Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis2010 Proteomics, Mar 9;10(10):1966-1984.
Broz AK, Broeckling CD, De-la-Peña C, Lewis MR, Greene E, Callaway RM, Sumner LW, Vivanco JM. "Plant neighbor identity influences plant biochemistry and physiology related to defense." BMC Plant Biol. 2010 Jun 17;10:115.
Mensack MM, Fitzgerald VK, Lewis MR, Thompson HJ. "Characterization of low molecular weight chemical fractions of dry bean (Phaseolus vulgaris) for bioactivity using Caenorhabditis elegans longevity and metabolite fingerprinting." J Agric Food Chem. 2010 Jun 9;58(11):6697-705.
Wamsley, S.; Broeckling, C.; Hess, A.; Prenni, J.; Curthoys, N. "Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules" 2010 Am J Physiol Renal Physiol, 298(6):F1323-31.
Mueller, N.H.; Walters, M.S.; Marcus, R.; Graf, L.L.; Prenni, J.E.; Gilden, D.; Silverstein, S.J.; Cohrs, R.J. “Identification of Phosphorylated Residues on Varicella-Zoster Virus Immediate Early Protein ORF63” 2010, J Gen Virol, May;91(Pt 5):1133-7.
Wallenstein, M.D.; Hess, A.M.; Lewis, M.R.; Steltzer, H.;Ayres, E. "Decomposition of aspen leaf litter results in unique metabolomes when decomposed under different tree species." Soil Biology & Biochemistry, Jan 2010, 1-7.
Olver, C.S; Webb, T.L; Long, L.J.; Scherman, H.; Prenni, J.E. “Comparison of methods for albumin and immunoglobulin depletion from equine serum.” Vet Clin Pathol. 2010 Aug 18.
Bhamidi, S.; Scherman, M.S.; Rithner, C.D.; Prenni, J.E.; Chatterjee, D.; Khoo, K.; and McNeil, M.R. “The Identification and Location of Succinyl Residues and the Characterization of the Interior Arabinan Region Allows for a Model of the Complete Primary Structure of Mycobacterium Tuberculosis Mycolyl Arabinogalactan” Journal of Biological Chemistry, Feb 2008.
Dooley, G.P.; Reardon, K.F.; Prenni, J.E.; Legare, M.E.; Foradori, C.D.; Tessari, J.E.; and Hanneman, W.H. “Proteomic Analysis of Diaminochlorotriazine (DACT) Adducts in Wister Rat Pituitary Glands and LbT2 Rat Pituitary Cells” Chemical Research in Toxicology 2008.
Prenni, J.E.; Avery, A.C.; Olver, C.S. "Proteomics: A review and an example using the reticulocyte membrane proteome." Veterinary Clinical Pathology, 2007, 36(1) 13-24.
Lyons, T.R.; Thorburn, J.; Ryan, P.W.; Thorburn, A.; Anderson, S.M.; Kassenbrock, C.K. "Regulation of the Pro-apoptotic Scaffolding Protein POSH by Akt " Journal of Biological Chemistry, 2007, 282(30), 21987-21997 .
Dooley, G.P.; Prenni, J.E.; Cranmer, B.K.; Prentiss P.L.; Andersen, M.E.; Tessari, J.D. "Identification of a Novel Hemoglobin Adduct in Sprague Dawley Rats Exposed to Atrazine" Chemical Research In Toxicology, 2006 , 19(5), 692-700.
Eckstein, T.M.; Chandrasekaran, S.; Mahapatra, S.; McNeil, M.R.; Chatterjee, D.; Rithner, C.D.; Ryan, P.W.; Belisle, J.T.; Inamine, J.M. "A Major Cell Wall Lipopeptide of Mycobacterium avium subspecies paratuberculosis" J. Biol. Chem. , 2006 , 281(8), 5209-5215.